apr 003 alomone  (Alomone Labs)

Journal: Journal of Tissue Engineering

Article Title: Optogenetically modified human embryonic stem cell-derived otic neurons establish functional synaptic connection with cochlear nuclei

doi: 10.1177/20417314241265198

Figure Lengend Snippet: Primary antibody list.

Article Snippet: P2X2 Receptor , Rabbit , APR-003 Alomone , 1/200.

Techniques:

Journal: ERJ Open Research

Article Title: Variability in P2X receptor composition in human taste nerves: implications for treatment of chronic cough

doi: 10.1183/23120541.00007-2023

Figure Lengend Snippet: Primary and secondary antisera

Article Snippet: Most samples were stained using the Alomone antibody to P2X2 (AB_2040054).

Techniques: Transduction

Journal: ERJ Open Research

Article Title: Variability in P2X receptor composition in human taste nerves: implications for treatment of chronic cough

doi: 10.1183/23120541.00007-2023

Figure Lengend Snippet: Fungiform taste buds. P2X2 and P2X3 receptor staining in a–c) mouse, d–f) rhesus macaque monkey and g–i) human. In all panels, a subset of type II taste cells is marked by GNAT3 (red). a, d, g) Across all species, Tuj1 (green) marks both intra- and perigemmal nerve fibres in and around taste buds. b, e, h) P2X2 (green) staining in the same taste buds; P2X2 immunoreactivity is present in b) mouse but not e) monkey or h) human taste buds. c, f, i) An adjacent section with the presence of P2X3 staining (green) in all species. Solid arrows indicate P2X receptor-positive nerve fibres. Empty arrows indicate an absence of P2X2-stained fibres and match the position of the solid arrows indicating positively stained nerve fibres in the left column. Insets in the right column show colocalisation between Tuj1 and P2X3, which is present in all samples. Colocalisation is quantified by the PDM (product of differences from the mean) image, which indicates colocalisation of Tuj1 and P2X3 or Tuj1 and P2X2 via fluorescence intensity and by pixel, i.e. location. PDM values are pseudo-coloured such that blue=modest colocalisation, red=higher, white=highest and black=below average. For all species, Tuj1 and P2X3 show higher to highest colocalisation throughout the taste bud. In mice, Tuj1 and P2X2 also show higher to highest colocalisation. Scale bars: 10 μm for all panels, including insets. Tuj1: β-tubulin III (a marker for neural processes); GNAT3: α-gustducin (a marker for some type II taste cells); DAPI: 4′,6-diamidino-2-phenylindole (a general nuclear stain).

Article Snippet: Most samples were stained using the Alomone antibody to P2X2 (AB_2040054).

Techniques: Staining, Fluorescence, Marker

Journal: ERJ Open Research

Article Title: Variability in P2X receptor composition in human taste nerves: implications for treatment of chronic cough

doi: 10.1183/23120541.00007-2023

Figure Lengend Snippet: Laryngeal taste buds. P2X2 and P2X3 receptor staining in a–c) mouse, d–f) rhesus macaque monkey and g–i) human. In all panels, a subset of type II taste cells is marked by GNAT3 (red). a, d, g) Across all species, Tuj1 (green) marks both intra- and perigemmal nerve fibres in taste buds. b, e, h) P2X2 (green) staining in the same taste buds; P2X2 immunoreactivity is present in b) mouse but not e) monkey or h) human taste buds. c, f, i) An adjacent section with the presence of P2X3 staining (green) in all species. Solid arrows indicate P2X receptor-positive nerve fibres. Empty arrows indicate an absence of P2X2-stained fibres and match the position of the solid arrows indicating positively stained nerve fibres in the left column. Insets in the right column show colocalisation between Tuj1 and P2X3, which is present in all samples. Colocalisation is quantified by the PDM (product of differences from the mean) image, which indicates colocalisation of Tuj1 and P2X3 or Tuj1 and P2X2 via fluorescence intensity and by pixel, i.e. location. PDM values are pseudo-coloured such that blue=modest colocalisation, red=higher, white=highest and black=below average. For all species, Tuj1 and P2X3 show higher to highest colocalisation throughout the taste bud. In mice, Tuj1 and P2X2 also show higher to highest colocalisation. Scale bars: 10 μm for all panels, including insets. Tuj1: β-tubulin III (a marker for neural processes); GNAT3: α-gustducin (a marker for some type II taste cells); DAPI: 4′,6-diamidino-2-phenylindole (a general nuclear stain).

Article Snippet: Most samples were stained using the Alomone antibody to P2X2 (AB_2040054).

Techniques: Staining, Fluorescence, Marker

Journal: ERJ Open Research

Article Title: Variability in P2X receptor composition in human taste nerves: implications for treatment of chronic cough

doi: 10.1183/23120541.00007-2023

Figure Lengend Snippet: Human taste buds showing immunoreactivity for P2X2. a–c) Three subjects in the adult fungiform cohort from the USA group demonstrated positive staining for b) P2X2 as well as for c) P2X3 and a) Tuj1. d–f) One subject in the paediatric laryngeal group demonstrated positive staining for e) P2X2 as well as for f) P2X3 and d) Tuj1. Scale bar: 10 μm for all panels. Tuj1: β-tubulin III (a marker for neural processes); GNAT3: α-gustducin (a marker for some type II taste cells); DAPI: 4′,6-diamidino-2-phenylindole (a general nuclear stain).

Article Snippet: Most samples were stained using the Alomone antibody to P2X2 (AB_2040054).

Techniques: Staining, Marker

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques:

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Western blot analysis of P2X receptor expression in the anterior pituitary. a Lane P2X1 to P2X7 are the results detected by P2X1 to P2X7 antibodies, respectively, lane M is the molecular weight marker, note that a strong immunostained band was detected by the P2X4 antibody, followed by the P2X5, P2X3, P2X2, P2X7, and P2X6 receptor antibodies, no band was detected by P2X1 receptor antibody. b Lane P2X1 to P2X7 are the results of the antibody pre-absorption control experiments for P2X1 to P2X7, note that no bands were detected. c The band density ratio of each P2X receptor over GAPDH

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: Western Blot, Expressing, Molecular Weight, Marker, Control

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Expression of P2X1 to P2X7 receptor-ir (red) in the rat anterior pituitary. a to f P2X1 to P2X7 receptor immunoreactive cells respectively. Note that strong immunostaining was detected using the P2X4 receptor antibody, followed by P2X5, P2X3, P2X6, P2X2, and P2X7 receptor antibodies. No obvious immunostaining was detected by the P2X1 antibody. g A merged image from f and an image of Iba-1-ir cells (green) (a macrophage/microglia marker) in the same field of f. Note that all the P2X7 receptor reactive cells were labeled by Iba-1-ir. All scale bars = 120 μm

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: Expressing, Immunostaining, Marker, Labeling

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Colocalization of P2X2 receptor-ir (green) with ACTH-ir, LH-ir, TSH-ir (red), and GH and PRL mRNA hybridization signals (red) in rat anterior pituitary. Note that majority of P2X2 receptor-ir cells were labeled by S100-ir f, no P2X2-ir cells were labeled by ACTH-ir, LH-ir, TSH-ir, and GH and PRL mRNA hybridization signals. An arrow indicates a cell double labeled (yellow) with P2X2-ir and S100-ir. All scale bars = 120 μm

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: Hybridization, Labeling

Journal: Purinergic Signalling

Article Title: Expression of P2X receptors in the rat anterior pituitary

doi: 10.1007/s11302-019-09685-y

Figure Lengend Snippet: Coexistence between P2X receptors and pituitary hormones or S100

Article Snippet: Table shows the primary antibodies used in this study. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Primary antibodies Host company Catalog no. Dilution IF TSA WB P2X1 Rabbit Alomone APR-001 1:200 1:4000 1:1000 P2X2 Rabbit Alomone APR-003 1:200 1:4000 1:1000 P2X3 Rabbit Roche 1:400 1:8000 1:1000 P2X4 Rabbit Santa Cruz ARP-002 1:400 1:8000 1:1000 P2X5 Rabbit Boster PB0339 1:500 1:8000 1:1000 P2X6 Rabbit Alomone APR-013 1:200 1:4000 1:1000 P2X7 Rabbit Alomone APR-004 1:400 1:8000 1:1000 ACTH Rabbit Sigma A1927 1:600 1:10000 TSH Rabbit Chemicon AB976 1:400 1:10000 S-100 Mouse Abcam ab4066 1:200 Iba-1 Rabbit Wako 17919741 1:1000 Digoxigenin Mouse Jackson 200–002-156 1:200 GAPDH Mouse Beyotime AG019 1:1000 Open in a separate window Primary antibodies used in this study As most of the primary antibodies are from the same host (rabbit), the double- or triple-labeling immunofluorescence protocol was used and modified based on previous reports [ 11 , 15 ].

Techniques: